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mspi  (New England Biolabs)


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    Structured Review

    New England Biolabs mspi
    Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mspi+methyltransferase/MspI+Methyltransferase/pm41825606-108-13-20
    Average 94 stars, based on 24 article reviews
    mspi - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Metabolic engineering of Clostridium cellulolyticum for the production of n -butanol from crystalline cellulose
    Article Snippet: .. The pM9 vector was methylated with the MspI methyltransferase from Moraxella sp. ATCC 49670 (NEB) and then introduced into C. cellulolyticum by electroporation, with successful transformation confirmed by plasmid rescue. ..

    Article Title: Target integration of an exogenous β-glucosidase enhances cellulose degradation and ethanol production in Clostridium cellulolyticum.
    Article Snippet: • First study to integrate an exogenous gene into Clostridium cellulolyticum.. • Integration of secretory β-glucosidase increases cellulose degradation

    Article Title: Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol yield from cellulose and switchgrass fermentations
    Article Snippet: .. The plasmid DNA was treated with MspI methyltransferase (New England Biolabs, Ipswich, MA) for 3 h, followed by purification with the DNA Clean and Concentrator Kit (Zymo Research, Irvine, CA). ..

    Article Title: Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes in Clostridium cellulolyticum
    Article Snippet: .. Plasmid DNA was methylated with MspI methyltransferase (NEB), followed by DNA purification and quantification. .. For each transformation, a 100 μl cell suspension was mixed with 2.0 μg of methylated plasmids and then electroporated in a 2-mm cuvette (1.25 kV, 5 ms, 1 square pulse) with a Gene Pulser Xcell (Bio-Rad) in the anaerobic chamber.

    Methylation:

    Article Title: Metabolic engineering of Clostridium cellulolyticum for the production of n -butanol from crystalline cellulose
    Article Snippet: .. The pM9 vector was methylated with the MspI methyltransferase from Moraxella sp. ATCC 49670 (NEB) and then introduced into C. cellulolyticum by electroporation, with successful transformation confirmed by plasmid rescue. ..

    Article Title: Target integration of an exogenous β-glucosidase enhances cellulose degradation and ethanol production in Clostridium cellulolyticum.
    Article Snippet: • First study to integrate an exogenous gene into Clostridium cellulolyticum.. • Integration of secretory β-glucosidase increases cellulose degradation

    Article Title: Metabolic engineering of Clostridium cellulolyticum for the production of n -butanol from crystalline cellulose
    Article Snippet: The competent cells were mixed with 1–5 μg of in vitro methylated DNA and transferred to a 0.2-mm gap cuvette (BioRad, Hercules, CA, USA) before electroporation with a BioRad Micropulser set at 1.5 kV. .. DNA was methylated with MspI methyltransferase (NEB) for at least 4 h according to the manufacturer’s instructions. ..

    Article Title: Weight loss after gastric bypass surgery in human obesity remodels promoter methylation.
    Article Snippet: .. The external cytosine residue of the sequence CCGG was methylated by MspI methyltransferase (New England BioLabs) supplemented with fresh S-adenosylmethionine. ..

    Article Title: Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes in Clostridium cellulolyticum
    Article Snippet: .. Plasmid DNA was methylated with MspI methyltransferase (NEB), followed by DNA purification and quantification. .. For each transformation, a 100 μl cell suspension was mixed with 2.0 μg of methylated plasmids and then electroporated in a 2-mm cuvette (1.25 kV, 5 ms, 1 square pulse) with a Gene Pulser Xcell (Bio-Rad) in the anaerobic chamber.

    Electroporation:

    Article Title: Metabolic engineering of Clostridium cellulolyticum for the production of n -butanol from crystalline cellulose
    Article Snippet: .. The pM9 vector was methylated with the MspI methyltransferase from Moraxella sp. ATCC 49670 (NEB) and then introduced into C. cellulolyticum by electroporation, with successful transformation confirmed by plasmid rescue. ..

    Transformation Assay:

    Article Title: Metabolic engineering of Clostridium cellulolyticum for the production of n -butanol from crystalline cellulose
    Article Snippet: .. The pM9 vector was methylated with the MspI methyltransferase from Moraxella sp. ATCC 49670 (NEB) and then introduced into C. cellulolyticum by electroporation, with successful transformation confirmed by plasmid rescue. ..

    Article Title: Target integration of an exogenous β-glucosidase enhances cellulose degradation and ethanol production in Clostridium cellulolyticum.
    Article Snippet: • First study to integrate an exogenous gene into Clostridium cellulolyticum.. • Integration of secretory β-glucosidase increases cellulose degradation

    Purification:

    Article Title: Target integration of an exogenous β-glucosidase enhances cellulose degradation and ethanol production in Clostridium cellulolyticum.
    Article Snippet: • First study to integrate an exogenous gene into Clostridium cellulolyticum.. • Integration of secretory β-glucosidase increases cellulose degradation

    Article Title: Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol yield from cellulose and switchgrass fermentations
    Article Snippet: .. The plasmid DNA was treated with MspI methyltransferase (New England Biolabs, Ipswich, MA) for 3 h, followed by purification with the DNA Clean and Concentrator Kit (Zymo Research, Irvine, CA). ..

    Residue:

    Article Title: Weight loss after gastric bypass surgery in human obesity remodels promoter methylation.
    Article Snippet: .. The external cytosine residue of the sequence CCGG was methylated by MspI methyltransferase (New England BioLabs) supplemented with fresh S-adenosylmethionine. ..

    Sequencing:

    Article Title: Weight loss after gastric bypass surgery in human obesity remodels promoter methylation.
    Article Snippet: .. The external cytosine residue of the sequence CCGG was methylated by MspI methyltransferase (New England BioLabs) supplemented with fresh S-adenosylmethionine. ..

    Inhibition:

    Article Title: A possible role of CTV.20 gene methylation in response to Citrus tristeza virus infection
    Article Snippet: Citrus tristeza virus (CTV) is the pathogen causing tristeza diseases in several Rutaceae species and leading to significant economic damage to citrus worldwide.. The Ctv locus provides broad spectrum resistance to CTV in Poncirus trifoliata L. Raf.. This locus is present also in tolerant and susceptible species, so an epigenetic mechanism of Ctv expression regulation was proposed.

    DNA Purification:

    Article Title: Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes in Clostridium cellulolyticum
    Article Snippet: .. Plasmid DNA was methylated with MspI methyltransferase (NEB), followed by DNA purification and quantification. .. For each transformation, a 100 μl cell suspension was mixed with 2.0 μg of methylated plasmids and then electroporated in a 2-mm cuvette (1.25 kV, 5 ms, 1 square pulse) with a Gene Pulser Xcell (Bio-Rad) in the anaerobic chamber.



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